Search Patents
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High resolution imaging microscope (HIRIM) and uses thereof
Patent number: 5859700
Abstract: The invention pertains to a high resolution imaging microscope, and more specifically, to a digital imaging spectrophotometer (DIS) configured to enable the direct determination of the ground state absorption spectra of microscopic samples from violet to near infrared. The instrument is capable of simultaneously recording hundreds of spectra, and can, using image processing techniques identify features (such as a single microbial cell) on a microscope slide or thin section. The invention additionally relates to the use of such an instrument.
Type: Grant
Filed: November 22, 1995
Date of Patent: January 12, 1999
Assignee: Kairos Scientific, Inc.
Inventor: Mary M. Yang
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High resolution imaging microscope (HIRIM) and uses thereof
Patent number: 6160617
Abstract: The invention pertains to a high resolution imaging microscope, and more specifically, to a digital imaging spectrophotometer (DIS) configured to enable the direct determination of the ground state absorption spectra of microscopic samples from violet to near infrared. The instrument is capable of simultaneously recording hundreds of spectra, and can, using image processing techniques identify features (such as a single microbial cell) on a microscope slide or thin section. The invention additionally relates to the use of such an instrument.
Type: Grant
Filed: January 12, 1999
Date of Patent: December 12, 2000
Assignee: Kairos Scientific, Inc.
Inventor: Mary M. Yang
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Solid phase enzyme kinetics screening in microcolonies
Patent number: 6472163
Abstract: Improvements in calorimetric assays, surfaces for arraying microcolonies and instrument hardware for screening mutagenized enzymes and proteins in a solid phase format are presented. These improvements permit new enzyme activities to be screened. New filter membrane materials and formats for arraying the microcolonies provide higher throughput, better solvent resistance and ease of handling. Modifications to the instrument heating and illumination systems provide improved temperature control and a more compact, folded light path.
Type: Grant
See AlsoMapleStory Reboot Server Guide - DigitalTQ2024 MapleStory Summer Showcase- Milestone Update Summary!Cannabis farming systems and methodsFiled: March 7, 2000
Date of Patent: October 29, 2002
Assignee: Kairos Scientific, Inc.
Inventors: William J. Coleman, Edward J. Bylina, Douglas C. Youvan
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Multispectral taxonomic identification
Patent number: 6738502
Abstract: The present invention provides an instrument and methods for a multispectral optical technique that can simultaneously classify individual biological cells within mixed populations. This invention, known as Multispectral Taxonomic Identification (MTID), shows that microscopy can be combined with a software analysis program to measure and categorize the fluorescence and other spectroscopically identifiable signals from complex populations of cells in situ, without cultivation. The invention thus enables high-throughput screening of cells for taxonomic classification.
Type: Grant
Filed: June 2, 2000
Date of Patent: May 18, 2004
Assignee: Kairos Scientific, Inc.
Inventors: William Coleman, Michael A. Tanner, Christopher M. Silva, Edward Bylina, Steven J. Robles, Michael R. Dilworth, Douglas C. Youvan, Mary M. Yang
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Solid phase enzyme kinetics screening in microcolonies
Patent number: 5914245
Abstract: A MicroColonyImager instrument and solid phase methods to screen cells expressing mutagenized enzymes for enhanced activity. The MicroColonyImager instrument and methods permit high throughput screening of enzyme libraries by time course analyses of single-pixels, using either absorption, fluorescence or FRET. This high throughput assay can detect small differences in enzyme rates within microcolonies grown at a nearly confluent density on an assay disk. Each microcolony is analyzed simultaneously at single-pixel resolution, requiring less than 100 ml substrate/measurement. By simultaneously assaying different substrates tagged with spectrally distinct chromogenic or fluorogenic reporters, the substrate specificity of an enzyme can be changed.
Type: Grant
Filed: June 16, 1998
Date of Patent: June 22, 1999
Assignee: Kairos Scientific Inc.
Inventors: Edward J. Bylina, William J. Coleman, Michael R. Dilworth, Christopher M. Silva, Mary M. Yang, Douglas C. Youvan
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Calibration of fluorescence resonance energy transfer in microscopy
Patent number: 6661909
Abstract: Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red).
Type: Grant
Filed: July 23, 2001
Date of Patent: December 9, 2003
Assignee: Kairos Scientific, Inc.
Inventors: Dougalas C. Youvan, Christopher M. Silva, Edward J. Bylina, William J. Coleman, Michael R. Dilworth, Mary M. Yang
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Calibration of fluorescence resonance energy transfer in microscopy
Patent number: 6456734
Abstract: Imaging hardware, software, calibrants, and methods are provided to visualize and quantitate the amount of Fluorescence Resonance Energy Transfer (FRET) occurring between donor and acceptor molecules in epifluorescence microscopy. The MicroFRET system compensates for overlap among donor, acceptor, and FRET spectra using well characterized fluorescent beads as standards in conjunction with radiometrically calibrated image processing techniques. The MicroFRET system also provides precisely machined epifluorescence cubes to maintain proper image registration as the sample is illuminated at the donor and acceptor excitation wavelengths. Algorithms are described that pseudocolor the image to display pixels exhibiting radiometrically-corrected fluorescence emission from the donor (blue), the acceptor (green) and FRET (red).
Type: Grant
Filed: June 5, 1998
Date of Patent: September 24, 2002
Assignee: Kairos Scientific, Inc.
Inventors: Douglas C. Youvan, Christopher M. Silva, Edward J. Bylina, William J. Coleman, Michael R. Dilworth, Mary M. Yang
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Optical instrument having a variable optical filter
Patent number: 5852498
Abstract: An optical instrument for use in collecting light from an object, the optical instrument having a post-objective waist with a variable optical filter positioned approximately at the post-objective waist. The invention also comprises an optical instrument for collecting light from an object, comprising an objective lens positioned to receive light from the object, an exit lens positioned to focus the light at a focal position, and a variable optical filter positioned between the objective lens and the focal position approximately at a waist of the light. The optical instrument may be an epifluorescence microscope for use in observing an image formed from light from an object under illumination by a light source. The light source may be, for example, a filtered light source or a monochromatic light source, preferably in an epifluorescence configuration. The variable optical filter may be a circular variable interference filter or a tilting interference filter.
Type: Grant
Filed: April 4, 1997
Date of Patent: December 22, 1998
Assignee: KAIROS Scientific Inc.
Inventors: Douglas C. Youvan, Mary M. Yang
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Visualization and Processing of Multidimensional Data Using Prefiltered and Sorting Criteria
Publication number: 20070217689
Abstract: Complex multidimensional datasets generated by digital imaging spectroscopy can be organized and analyzed by applying software and computer-based methods comprising sorting algorithms. Combinations of these algorithms to images and graphical data, allow pixels or features to be rapidly and efficiently classified into meaningful groups according to defined criteria. Multiple rounds of pixel or feature selection may be performed based on independent sorting criteria. In one embodiment sorting by spectral criteria (e.g., intensity at a given wavelength) is combined with sorting by temporal criteria (e.g., absorbance at a given time) to identify microcolonies of recombinant organisms harboring mutated genes encoding enzymes having desirable kinetic attributes and substrate specificity. Restriction of the set of pixels analyzed in a subsequent sort based on criteria applied in an earlier sort (“sort and lock” analyses) minimize computational and storage resources.
Type: Application
Filed: May 17, 2007
Publication date: September 20, 2007
Applicant: KAIROS-SCIENTIFIC INC.
Inventors: Mary Yang, Edward Bylina, William Coleman, Michael Dilworth, Steven Robles, Douglas Youvan
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Visualization and processing of multidimensional data using prefiltered and sorting criteria
Patent number: 7379601
Abstract: Complex multidimensional datasets generated by digital imaging spectroscopy can be organized and analyzed by applying software and computer-based methods comprising sorting algorithms. Combinations of these algorithms to images and graphical data, allow pixels or features to be rapidly and efficiently classified into meaningful groups according to defined criteria. Multiple rounds of pixel or feature selection may be performed based on independent sorting criteria. In one embodiment sorting by spectral criteria (e.g., intensity at a given wavelength) is combined with sorting by temporal criteria (e.g., absorbance at a given time) to identify microcolonies of recombinant organisms harboring mutated genes encoding enzymes having desirable kinetic attributes and substrate specificity. Restriction of the set of pixels analyzed in a subsequent sort based on criteria applied in an earlier sort (“sort and lock” analyses) minimize computational and storage resources.
Type: Grant
Filed: May 17, 2007
Date of Patent: May 27, 2008
Assignee: Kairos-Scientific Inc.
Inventors: Mary Mei-Iing Yang, Edward J. Bylina, William Joseph Coleman, Michael Robert Dilworth, Steven J. Robles, Douglas Charles Youvan
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Visualization and processing of multidimensional data using prefiltering and sorting criteria
Patent number: 6834122
Abstract: Complex multidimensional datasets generated by digital imaging spectroscopy can be organized and analyzed by applying software and computer-based methods comprising sorting algorithms. Combinations of these algorithms to images and graphical data, allow pixels or features to be rapidly and efficiently classified into meaningful groups according to defined criteria. Multiple rounds of pixel or feature selection may be performed based on independent sorting criteria. In one embodiment sorting by spectral criteria (e.g., intensity at a given wavelength) is combined with sorting by temporal criteria (e.g., absorbance at a given time) to identify microcolonies of recombinant organisms harboring mutated genes encoding enzymes having desirable kinetic attributes and substrate specificity. Restriction of the set of pixels analyzed in a subsequent sort based on criteria applied in an earlier sort (“sort and lock” analyses) minimize computational and storage resources.
Type: Grant
Filed: January 22, 2001
Date of Patent: December 21, 2004
Assignee: Kairos Scientific, Inc.
Inventors: Mary M. Yang, Edward J. Bylina, William J. Coleman, Michael R. Dilworth, Steven J. Robles, Douglas C. Youvan
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